HLA class II alleles associations of anticardiolipin and anti- 2GPI antibodies in a large series of European patients with systemic lupus erythematosus
Mauro Galeazzi1,
Gian Domenico Sebastiani2,
Angela Tincani3,
Jean-Charles Piette4,
Flavio Allegri3,
Gabriella Morozzi1,
Francesca Bellisai1,
Raffaella Scorza5,
Giovanni Battista Ferrara6,
Carlo Carcassi7,
Josep Font8,
Giuseppe Passiu9,
Josef Smolen10,
Chryssa Papasteriades11,
Frederic Houssiau12,
Antonio Fernandez Nebro13,
Enrique De Ramon Garrido14,
Anna Jedryka-Goral15,
Roberto Marcolongo1 & the European Concerted Action on the Immunogenetics of SLE16
1Istituto di Reumatologia, Università di Siena, Italy
2Divisione di Reumatologia, Ospedale San Camillo, Roma, Italy
3Servizio di Immunologia Clinica, Spedali Civili, Brescia, Italy
4Service Medecine Interne, CHU Pitié-Salpetrière, Paris, France
5Laboratorio di Immunologia Cellulare e Immunogenetica, Istituto di Medicina Interna, Malattie Infettive e Immunopatologia, Università di Milano, Italy
6Laboratorio di Immunogenetica, Centro di Biotecnole gie Avanz TE, Genova, Italy
7Dipartimento di Genetica Medica, Istituto di Clinica Medica, Università di Cagliari, Italy
8Unitat de Malalties Autoimmunes Sistèmiques, Hospital Clinic, Barcelona, Spain
9II Cattedra di Reumatologia, Dipartimento di Scienze Mediche, Centro Malattie Reumatiche Sistemiche, Università di Cagliari, Italy
102nd Department of Medicine, Center for Rheumatic Diseases, Lainz Hospital, Vienna, Austria
11Department of Immunology and Histocompatibility, Evangelismos Hospital, Athens, Greece
12Service du Rhumatologie, Cliniques Universitaires Saint-Luc Université Catholique de Louvain, Bruxelles, Belgium
13Departement of Medicine, Rheumatology Service, Section Lupus Unit, Universitary Hospital, University of Malaga, Spain
14Unidad de Enfermedades Autoimmunes Sistemicas, Hospital Regional del SAS, Servicio de Medicina Interna, Malaga, Spain
15Department of Connective Tissue Diseases, Institute of Rheumatology, Warsaw, Poland
Correspondence to: Professer Mauro Galeazzi
, Institute of Rheumatology – Policlinico "Le Scotte’’ – Viale Bracci, 53100 Siena, Italy.
16Participating centres are listed on page 55.
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The objective of this study was to determine the HLA class II associations of the anticardiolipin (aCL) and anti-2GPI (a2GPI) antibodies in a large series of European patients with systemic lupus erythematosus (SLE). A cohort of 577 European SLE patients was enrolled. aCL and a2GPI were measured by ELISA methods. Molecular typing of HLA-DRB1, DRB3, DRB4, DRB5, DQA1 and DQB1 loci was performed by the polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) method. aCL of IgG, IgM and IgA isotypes were detected in 22.8%, 14% and 13.9% of patients, respectively. IgG and IgM a2GPI were detected in 20% of patients. aCL showed positive association with HLA DRB1*04, DRB1*0402, DRB1*0403, DRB1*07, DRB3*0301, DQA1*0201, DQA1*0301, DQB1*0302, and negative association with DQA1*0501, DRB3*0202. a2GPI showed positive association with DRB1*0402, DRB1*0403, DQB1*0302. DRB1*0402 carried the highest relative risk for the presence of both aCL (RR=8.1) and a2GPI (RR=4.6). Our results confirm the already described associations of aCL with HLA DR4 and DR7, but also demonstrate that, among the alleles at the DRB1*04 locus, the *0402 was most represented both in aCL and in a2GPI positive patients. In addition, HLA class II associations of a2GPI are for the first time extensively examined in a large cohort of European SLE patients. Lupus (2000) 9, 47-55 |
