Modification of PDGF receptor expression or function alters the metastatic phenotype of 3LL cells
Cheryl J Fitzer-Attas1,2,
Myoung-Sool Do1,3,
Sara Feigelson1,
Ezra Vadai1,
Michael Feldman1 & Lea Eisenbach1,4
1Department of Immunology, The Weizmann Institute of Science, 76100 Rehovot, Israel
2G.W. Hooper Foundation, University of California, San Francisco, California
3Department of Biological Engineering, Handong University, Kyungbuk, Korea
4Author for correspondence CJF-A, M-SD and EV contributed equally to this work
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Functional PDGF receptors are selectively expressed on highly lung-metastasizing clones of the 3LL Lewis lung carcinoma, but not on low-mestastatic clones. The highly metastatic clones are also growth induced in vitro by PDGF and lung conditioned medium. To investigate whether modification of PDGF receptor expression or function can affect metastatic capability, we transfected cells of a low-metastatic 3LL clone with a full length PDGF receptor gene and cells of a highly-metastatic clone with a truncated kinase domain PDGF receptor gene. Introduction of the full length PDGF receptor conferred upon low-metastatic cells the ability to grow in vitro in the presence of PDGF-AA and to colonize the lung in experimental and spontaneous metastases assays. Conversely, introduction of a truncated version of the PDGF receptor into highly metastatic cells reduced their metastatic load to control levels. Accordingly, their responses to PDGF-AA, including growth stimulation and receptor autophosphorylation, were reduced. These results demonstrate that PDGF receptor expression and function can control the capacity of tumor cells to generate metastases in the lung. The response of this receptor to lung-derived PDGF-like factors may define a paracrine mode of metastatic cell growth in the target organ. |
