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G Hetet1, H Dastot2, M Baens3, A Brizard4, F Sigaux2, B Grandchamp1 & M-H Stern2
1Unité INSERM U409 and Centre de recherche Claude Bernard, Hôpital Bichat, Paris, France
2Unité INSERM U462, Laboratoire Associé n°10 du Comité de Paris de la Ligue Nationale Contre le Cancer, Institut Universitaire d’Hématologie, Hôpital Saint Louis, Paris, France
3Human Genome Laboratory, Center for Human Genetics, Flanders Interuniversity Institute for Biotechnology, University of Leuven, Leuven, Belgium
4Laboratoire Central d’Hématologie, CHRU de Poitiers, Poitiers, France
Correspondence to: M-H Stern, Unité INSERM U462, Centre Hayem, Institut Universitaire d’Hématologie, Hôpital Saint Louis, 75475 Paris Cedex 10, France; Tel: +33 1 53 72 22 15; Fax: +33 1 53 72 22 17; E-mail: mh.stern@chu-stlouis.fr
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Introduction: T-cell prolymphocytic leukemia is a rare form of mature leukemia which occurs in adults and in younger patients suffering ataxia telangiectasia. Among others, complex chromosome aberrations of chromosome 12 have been described in this disease. We searched for deletions of the 12p13 region as the result of these chromosome rearrangements. Material and methods: Paired leukemic and non-leukemic cells were obtained from a series of 21 patients suffering T-cell prolymphocytic leukemia. Loss of heterozygosity was searched for by microsatellite typing using a fluorescent automated laser DNA sequencer to analyze the amplification products. Proteins were analyzed by Western blot. Southern blot analysis of one patient was conducted. Results and conclusion: Loss of heterozygosity of the 12p13 region, including the ETV6 and CDKN1B genes, was detected in nine of these 21 cases (43%). Western and Southern blot analyses of one case demonstrated a biallelic deletion which did not include ETV6. Taken together, our results defined a minimal region of deletion of less than one Mb flanked by the markers b312C2T7 and D12S320, excluding ETV6 as a candidate gene. Deletion of the 12p13 region is thus a highly recurrent genetic event in T-cell prolymphocytic leukemia. The Hematology Journal (2000) 1, 42 – 47
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