January 2000, Volume 24, Issue 1, Pages 70 - 74

Paper

Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects J-P Bastard1,5, H Vidal2, C Jardel1,5, E Bruckert3,5, D Robin4, P Vallier2, P Blondy1, G Turpin3,5, C Forest4 & B Hainque1,5 1Service de Biochimie, Hôpital de la Salpêtrière, AP-HP, Paris     2INSERM U-449, Faculté de Médecine RTH Laënnec, Lyon     3Service d’Endocrinologie, Hôpital de la Pitié, AP-HP, Paris     4Centre de Recherche sur l’Endocrinologie Moléculaire et le Développement, CNRS, Meudon     5Institut Fédératif de Recherche Coeur, Muscle et Vaisseaux, Groupe Hospitalier Pitié-Salpêtrière, AP-HP, Paris, France     Correspondence to: J-P Bastard , Service de Biochimie (Pr. J. Delattre), Hôpital de la Salpêtrière, 47 boulevard de l’Hôpital, 75013, Paris, France. E-mail: jean-philippe.bastard@psl.ap-hop-paris.fr

Keywords

adipocyte;   obesity;   plasminogen activator inhibitor-1;   calorie restriction

Abstract

OBJECTIVE: To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-1 level during weight loss in obese humans. DESIGN: Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4.±1.9 kg/m2, aged 48±3 y, before and after a 3 week very low calorie diet (VLCD) programme (3.9±0.1 MJ/day). MEASUREMENTS: Plasma and adipose tissue PAI-1 protein levels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR. RESULTS: VLCD induced weight loss (5.8±0.8 kg) and decreased plasma PAI-1 concentration (-26% (P<0.01)). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P<0.05) and by 44% (P<0.01), respectively. CONCLUSION: These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects. International Journal of Obesity (2000)24, 70-74

Received 1 March 1999; Revised 18 June 1999; Accepted 27 July 1999

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