January 2001, Volume 25, Issue 1, Pages 75 - 83

Paper

Characterization of mouse GBP28 and its induction by exposure to cold M Yoda1, Y Nakano2, T Tobe3, S Shioda4, N-H Choi-Miura1 & M Tomita1 1Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan     2Pharmaceuticals and Medical Devices Evaluation Center, National Institute of Health Sciences, Tokyo, Japan     3Department of Medicinal Information, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan     4Department of Anatomy, School of Medicine, Showa University, Tokyo, Japan     Correspondence to: Y Nakano, Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. E-mail: yodamado@pharm.showa-u.ac.jp or E-mail: ynakano@nihs.go.jp

Keywords

adipocyte;   GBP28;   gelatin-binding;   high-fat diet;   fast;   cold

Abstract

OBJECTIVE: To investigate whether the expression of the novel adipose tissue-specific protein GBP28 in adipose tissue and serum are altered in mice under a variety of conditions. DESIGN: Mice were fed a high-fat diet for 4 weeks, fasted for 48 h or exposed at 4°C. SUBJECTS: C57BL/6J mouse, male, 4-6 weeks old. MEASUREMENTS: GBP28 mRNA, GBP28 protein, blood glucose, insulin and fad pad weight of the mice. RESULTS: We first confirmed that the mouse has GBP28 and its characteristics are the same as human GBP28. Serum concentration and mRNA levels of GBP28 significantly increased in the mice exposed to cold. CONCLUSION: GBP28 may play a role in homeostasis, regulating body temperature and basal metabolic rate in response to changing environmental conditions. International Journal of Obesity (2001) 25, 75-83

Received 21 January 2000; Revised 3 July 2000; Accepted 4 August 2000

^© Macmillan Publishers Ltd 2001